To monitor these events, the nanosized optical pH probes need to provide ratiometric signals in the optically transparent biological window, target to all relevant intracellular compartments, and to facilitate imaging at subcellular resolution without interference from the biological matrix. To meet these criteria we sensitize the surface conjugated pH sensitive indicator via an upconversion process utilizing an energy transfer from the nanoparticle to the indicator.
Live cells were imaged with a scanning confocal microscope equipped with a low-energy nm laser excitation, which facilitated high resolution and penetration depth into the specimen, and low phototoxicity needed for long-term imaging. Our upconversion nanoparticle resonance energy transfer based sensor with polyethylenimine-coating provides high colloidal stability, enhanced cellular uptake, and distribution across cellular compartments.
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This distribution was modulated with membrane integrity perturbing treatment that resulted into total loss of lysosomal compartments and a dramatic pH shift of endosomal compartments. These nanoprobes are well suited for detection of pH changes in in vitro models with high biological background fluorescence and in in vivo applications, e.
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Cited By. This article is cited by 31 publications. Michael S. Hilbers, Roxanne E. Kieltyka, Albert M. Brouwer, Marta M. Natile, Sylvestre Bonnet. Langmuir , 35 37 , DOI: Analytical Chemistry , 91 11 , Langmuir , 35 15 , Zheng Li, Jon R.
Askim, Kenneth S. Chemical Reviews , 1 , ACS Sensors , 3 11 , Analytical Chemistry , 90 14 , Kate J.
Carnevale, Ryan A. Riskowski, Geoffrey F. ACS Nano , 12 6 , Analytical Chemistry , 89 17 , ACS Nano , 11 4 , Fluorescent pH nanosensors: Design strategies and applications. ChemPhysChem , 20 8 , Journal of Luminescence , , International Journal of Molecular Sciences , 20 6 , Two-photon semiconducting polymer nanoparticles as a new platform for imaging of intracellular pH variation.
Biosensors and Bioelectronics , , Nanomaterials for Intracellular pH Sensing and Imaging. Development of near-infrared sensitized core—shell—shell upconverting nanoparticles as pH-responsive probes. If you do not receive an email within 10 minutes, your email address may not be registered, and you may need to create a new Wiley Online Library account.
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Learn more Check out. Abstract Biosensors, small devices enabling selective bioanalysis because of properly assembled biological recognition molecules, represent the fortuitous results of years of interdisciplinary and complementary investigations in different fields of science. Citing Literature. Related Information. Close Figure Viewer. Browse All Figures Return to Figure. Previous Figure Next Figure. Email or Customer ID.
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